Dowex AG50WX8 cation exchange resin 100 to 200mesh BioRad
The resin is prepared in large batches (200 to 400 ml packed resin) by the following series of washing steps. These washes can be conveniently performed using a large Buchner funnel and filter paper to collect the resin between changes of wash solution.
1. Wash resin in >10 vol of 0.5 N NaOH until no color is observed in wash solution (resin will retain its buff color).
4. Wash with 5 to 10 vol of distilled H2O.
6. Wash with distilled H2O until pH = 9.
7. Store prepared resin indefinitely in 0.5 M NaCl/0.1 M Tris, pH 7.5, at 4°C.
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