Critical Parameters Bfp
Successful peptide synthesis requires reagents of a quality appropriate to the particular step, and the careful application of those reagents. For example, the protected amino acids need to be free of reactive counterions such as dicyclohexylamine (DCHA), contaminating unprotected amino acid, isomers such as the D-amino acid, and water. Check carefully that the amino acid as supplied is EXACTLY the same as specified in the manual or on the software. Apart from quality testing each amino acid, the best assurance of quality is to buy only from reputable suppliers.
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