Derick Mussen Preventive Healthcare

Basic Footpad Injection Of The Mouse Protocol 9

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Materials

Injectate

1-ml syringe with 25-G needle

1. Manually restrain the animal (unit 1.3).

This procedure is usually done with one person restraining the animal while the other injects the substance.

2. Spread the toes of one hind foot and introduce the needle into the soft tissue pad of the plantar surface. Alternatively, the injection can be made subcutaneously between the second and third digits.

3. Inject no more than 50 ^l. Apply direct pressure following withdrawal of needle.

Parenteral Injections